laserbased micropipette puller p 2000 (Sutter Instrument Company)

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Sutter Instrument Company laserbased micropipette puller p 2000
Laserbased Micropipette Puller P 2000, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 180 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+2000+laser+based+micropipette+puller/P-2000%2FG+Laser-Based+Glass+Puller/10__1016_slash_j__pestbp__2026__107395-113-14-18
Average 96 stars, based on 180 article reviews

laserbased micropipette puller p 2000 - by Bioz Stars, 2026-11

96/100 stars

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Article Title: Separation and Detection of Charged Unilamellar Vesicles in Vacuum by a Frequency-Controlled Quadrupole Mass Sensor.
Article Snippet: Nanoelectrospray emitters were pulled with the P-2000 laser-based micropipette puller (Sutter Instrument, CA) from the 10 cm length 1 mm OD, 0.78 mm ID borosilicate glass with filament (Sutter Instrument, BF100− 78−10).

Article Title: Metal-induced nanoscale clusterization initiates protein liquid-liquid phase separation.
Article Snippet: The expression plasmids were constructed by insertion of yeast SUMO gene (Smt3) into a pET-28a vector using ClonExpress II One Step Cloning Kit (Vazyme), generating pET-28a-His 6 -SUMO and pET-28a-His 10 -TEV-SUMO plasmids for expression of His 6 -SUMO and SUMO proteins, respectively.

Article Title: In Vivo Electrochemical Aptasensors Reveal Reversible Dopamine Dysregulation from Adolescent Malnutrition.
Article Snippet: Dopamine (DA) signaling is essential for neurodevelopment and is particularly sensitive to disruption during adolescence.. Protein restriction (PR) can impair DA dynamics, yet mechanistic insights remain limited due to challenges in real-time neurochemical sensing.. Here, we present aptCFEDA3.0, a robust implantable aptamer-based sensor fabricated via a reagent-free, 3 min electrochemical conjugation (E-conjugation) using amine-quinone chemistry.

Injection:

Article Title: Beyond venomous fangs: Uloboridae spiders have lost their venom but not their toxicity
Article Snippet: .. Volumes of 46 nl were injected intrathoracically using glass capillaries pulled on a P-2000 Laser-Based Micropipette Puller (Sutter Instrument) held on a Nanoject II device (Drummond Scientific). .. Injections were performed under a Stemi 508 Stereomicroscope (Zeiss).

Article Title: Genome reorganisation and expansion shape 3D genome architecture and define a distinct regulatory landscape in coleoid cephalopods
Article Snippet: .. Injection needles were pulled on a P-2000 Laser-based Micropipette puller (settings: heat 700, filament 4, delay 140, pull 175; Sutter Instruments) using quartz capillaries (OD=1.0mm, ID=0.7mm, OL=10cm; Sutter Instruments, QF100-70-10) and then beveled at a 25° angle for 30 seconds on a BV-10 Microelectrode Beveler (Sutter instruments) to generate a 3-4 μm wide tip opening. ..

Article Title: Beyond venomous fangs: Uloboridae spiders have lost their venom but not their toxicity.
Article Snippet: .. Volumes of 46 nl were injected intrathoracically using glass capillaries pulled on a P-2000 Laser-Based Micropipette Puller (Sutter Instrument) held on a Nanoject II device (Drummond Scientific). .. Injections were performed under a Stemi 508 Stereomicroscope (Zeiss).

Glass:

Article Title: Ultrasensitive and Selective Iontronic H 2 O 2 Nanosensor Reveals the Biphasic Redox Dynamics Invoked by l-DOPA at a Single-Cell Level.
Article Snippet: As the gold-standard therapy for Parkinson’s disease, 3,4-dihydroxyphenylalanine (L-DOPA) alleviates motor symptoms but paradoxically induces concentrationdependent dyskinesia and oxidative stress due to redox dynamics involving hydrogen peroxide (H2O2) generation.. While vitamin B6 (VB6) modulates L-DOPA metabolism and redox balance, its pharmacodynamic interplay with L-DOPA remains controversial, hindered by methodological limitations in resolving intracellular H2O2 dynamics.. Here, we developed an ultrasensitive H2O2 iontronic nanosensor by integrating a hydrogel-filled nanopipette and a dual-amplification strategy, enabling in situ monitoring of dynamic redox changes induced by L-DOPA and VB6.

Produced:

Article Title: Proximity biotinylation at the host-Shigella interface reveals UFMylation as an antibacterial pathway
Article Snippet: Peptides were trapped on a 5 mm trapping column (Thermo Fisher Scientific, Pepmap, 300 μm internal diameter (I.D.), 5 μm beads) in a solvent with 0.1% TFA in water. .. Peptides were separated on an in-house produced column (75 μm x 100mm) with a laser-pulled electrospray tip using a P-2000 Laser-Based Micropipette Puller (Sutter Instruments). .. The column was packed with ReproSil Saphir 100 1.5 μm silica particles (Dr. Maisch, #ra115.9e).

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